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gst proteins  (MedChemExpress)
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MedChemExpress gst proteins
Gst Proteins, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gst bacterial expression vector pgex 6p 1  (Cytiva Europe)
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Gst Bacterial Expression Vector Pgex 6p 1, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gst bacterial expression vector pgex 6p 1 - by Bioz Stars, 2026-09
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e18 gst ncegt1  (Kaneka Corp)
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Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
E18 Gst Ncegt1, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/e18+gst+ncegt1/pmc13273134-159-21-8
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e18 gst ncegt1 - by Bioz Stars, 2026-09
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glutathione sepharose high performance  (Cytiva Europe)
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Cytiva Europe glutathione sepharose high performance
Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
Glutathione Sepharose High Performance, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/Glutathione+Sepharose+High+Performance/custom%4017527901%4041833707
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glutathione sepharose high performance - by Bioz Stars, 2026-09
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gst  (Kaneka Corp)
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Kaneka Corp gst
Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
Gst, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/gst+tmem209/pmc12967149-229-5-7
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gst - by Bioz Stars, 2026-09
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gst resin  (Brickell Biotech)
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Brickell Biotech gst resin
Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
Gst Resin, supplied by Brickell Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/ni+nta+resin+sefinose/pm42241470-276-14-17
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gst resin - by Bioz Stars, 2026-09
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anti gst  (Abmart Inc)
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Abmart Inc anti gst
Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
Anti Gst, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/anti+igg/pm42277594-145-3-6
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gst tag  (Covance)
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Covance gst tag
Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
Gst Tag, supplied by Covance, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/gst+tag/pm42248824-77-3-0
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gst tag - by Bioz Stars, 2026-09
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gst tagged protein  (Cytiva Europe)
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Cytiva Europe gst tagged protein
Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
Gst Tagged Protein, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/Glutathione+Sepharose+4B/10__1186_slash_s42483___026___00430___z-195-12-21
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gst tagged protein - by Bioz Stars, 2026-09
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gst gravitrap cytiva  (Cytiva Europe)
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Cytiva Europe gst gravitrap cytiva
Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
Gst Gravitrap Cytiva, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/GST+GraviTrap/pm42229414-748-272-274
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gst gravitrap cytiva - by Bioz Stars, 2026-09
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Effects of solubility tags and stability mutations on NcEGT1 expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 kDa), GST-NcEGT1 (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.

Journal: Synthetic and Systems Biotechnology

Article Title: Metabolic engineering of Saccharomyces cerevisiae for co-production of ergothioneine, salidroside and gadusol

doi: 10.1016/j.synbio.2026.04.010

Figure Lengend Snippet: Effects of solubility tags and stability mutations on NcEGT1 expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 kDa), GST-NcEGT1 (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.

Article Snippet: Among the constructed variants, a 33% increase in EGT production was observed in mutant E26 (GST-NcEGT1 A458P ) over that of E18 (GST-NcEGT1) ( D).

Techniques: Solubility, Expressing, Recombinant, Staining, SDS Page, Molecular Weight, Marker, Mutagenesis